利用斑马鱼模型评价化妆品祛斑作用

【评价原理】

色斑的形成与皮肤中黑色素的沉着有关，祛斑功效实质上是对黑色素的清除作用。通过斑马鱼技术能简洁而有效的评价化妆品的祛斑功效。

斑马鱼在发育初期全身透明，胚胎发育至 24 h时黑色素开始从视网膜上皮生长，48h逐渐形成稳定并可见的黑色素色斑。将受试物于斑马鱼发育48h时加入鱼水中，72h时观察黑色素的降低程度，从而评价祛斑功效。

【实验方案】

斑马鱼分成若干组，含正常对照组和供试品组。正常对照组不做处理，供试品组使用受试化妆品，孵育一段时间后，对斑马鱼头部进行拍照，利用高级图像处理软件分析斑马鱼头部的黑色素信号强度，从而评价其祛斑功效。

【评价指标】

  皮肤黑色素信号强度

【结果展示】（展示图片仅供参考，实际实验组别依据合同而定）

图1. 斑马鱼头部黑色素表型图（蓝色为分析区域）

【评价结论】

1、经过各组斑马鱼对比实验，阳性对照组和化妆品组与正常对照组对比，斑马鱼头部只有少量的黑色素。

2、本实验证实了该化妆品具有祛斑作用。

【参考文献】

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